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 Evaluation slide (TEST SLIDE)
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 Category Human tissue array
   Cat. No. BA
   Product description Evaluation slide (TEST SLIDE)
   Data sheet BA3
BA3
   Images
  general_information
   No. of samples 24
   Text
Current version: BA3
No. of normal cores: 12 cores
No. or cancer cores: 12 cores
The price of the TEST SLIDE is one-fourth of regular array slide. The quality of this test slide is same as the other array slides. This slide can be used for adjustment of experimental condition. The TEST SLIDE is also useful for most researchers who consider the experiment using tissue array slides.

Price : 5 slides for 600 €

BA3,  Test slide (normal organs and tumors)
       
No. Age Sex Organ Diagnosis
1 36 F skin normal
2 37 F breast normal
3 63 M spleen normal
4 67 M skeletal muscle normal
5 60 M lung normal
6 42 F liver normal
 
7 41 M stomach , body normal
8 53 M colon normal
9 35 M kidney normal
10 70 M prostate normal
11 30 F placenta normal
12 0 F brain normal
 
13 26 F breast infiltrating duct carcinoma
14 59 M lung squamous cell carcinoma
15 54 M liver hepatocellular carcinoma
16 52 M esophagus squamous cell carcinoma
17 49 M stomach adenocarcinoma
18 39 M small bowel malignant stromal tumor
 
19 57 F rectum adenocarcinoma
20 44 F kidney renal cell carcinoma
21 56 F urinary bladder transitiomal cell carcinoma
22 67 F uterus endometrial carcinoma
23 34 F ovary mucinous cystadenocarcinoma
24 64 F lymph node metastatic malignant melanoma
         
 
       
         
         
         
         
         
         

 

 

General Information of the Tissue Array Slide

Applications

bullet􀁺 Immunohistochemistry
bullet􀁺 In situ hybridization, RNA or DNA
bullet􀁺 Fluorescent in situ hybridization (FISH)
bullet􀁺 In situ PCR, RNA or DNA
bullet􀁺 TUNEL for apoptosis (Recommended procedures in www.tissue-array.com -> protocol) Storage

and stability

bullet􀁺 Individual slide is paraffin-coated and put in an air-tight pack with inert gas.
bullet􀁺 Tissue array slides are shipped in room temperature.
bullet􀁺 If the slides are stored in 4 degree, they are good up to one year.

How processed

bullet􀁺 Tissues were initially fixed with formalin except for some of the animal tissues.
bullet􀁺 Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
bullet􀁺 Cleared by xylene, three changes for 1 hour each.
bullet􀁺 Infiltrated with 60°C paraffin, three changes for 1 hour each
bullet􀁺 Microtoming in 4 µm thickness and put on silane coating slide from Muto Pure Chemicals (Japan)

Before use

bullet􀁺 Dry slides for 1 hour in a oven at 60 degree.
bullet􀁺 Dewax slides in xylene for 4 minutes x 5 times
bullet􀁺 Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
bullet􀁺 Immerse slides in tap water for 5 minutes

Slide orientation

bullet􀁺 Refer to data sheet for slide orientation.

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