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Price : 5 slides for 600 €
| BA3, Test slide (normal organs and tumors) |
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| No. |
Age |
Sex |
Organ |
Diagnosis |
| 1 |
36 |
F |
skin |
normal |
| 2 |
37 |
F |
breast |
normal |
| 3 |
63 |
M |
spleen |
normal |
| 4 |
67 |
M |
skeletal muscle |
normal |
| 5 |
60 |
M |
lung |
normal |
| 6 |
42 |
F |
liver |
normal |
| |
| 7 |
41 |
M |
stomach , body |
normal |
| 8 |
53 |
M |
colon |
normal |
| 9 |
35 |
M |
kidney |
normal |
| 10 |
70 |
M |
prostate |
normal |
| 11 |
30 |
F |
placenta |
normal |
| 12 |
0 |
F |
brain |
normal |
| |
| 13 |
26 |
F |
breast |
infiltrating duct carcinoma |
| 14 |
59 |
M |
lung |
squamous cell carcinoma |
| 15 |
54 |
M |
liver |
hepatocellular carcinoma |
| 16 |
52 |
M |
esophagus |
squamous cell carcinoma |
| 17 |
49 |
M |
stomach |
adenocarcinoma |
| 18 |
39 |
M |
small bowel |
malignant stromal tumor |
| |
| 19 |
57 |
F |
rectum |
adenocarcinoma |
| 20 |
44 |
F |
kidney |
renal cell carcinoma |
| 21 |
56 |
F |
urinary bladder |
transitiomal cell carcinoma |
| 22 |
67 |
F |
uterus |
endometrial carcinoma |
| 23 |
34 |
F |
ovary |
mucinous cystadenocarcinoma |
| 24 |
64 |
F |
lymph node |
metastatic malignant melanoma |
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General Information of the Tissue Array Slide
Applications
- Immunohistochemistry
- In situ hybridization, RNA or DNA
- Fluorescent in situ hybridization (FISH)
- In situ PCR, RNA or DNA
- TUNEL for apoptosis (Recommended procedures in www.tissue-array.com
-> protocol) Storage
and stability
- Individual slide is paraffin-coated and put in an air-tight pack with
inert gas.
- Tissue array slides are shipped in room temperature.
- If the slides are stored in 4 degree, they are good up to one year.
How processed
- Tissues were initially fixed with formalin except for some of the
animal tissues.
- Then, dehydrated with gradient ethanol; typically 1 hour each
progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
- Cleared by xylene, three changes for 1 hour each.
- Infiltrated with 60°C paraffin, three changes for 1 hour each
- Microtoming in 4 µm thickness and put on silane coating slide from
Muto Pure Chemicals (Japan)
Before use
- Dry slides for 1 hour in a oven at 60 degree.
- Dewax slides in xylene for 4 minutes x 5 times
- Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times
each.
- Immerse slides in tap water for 5 minutes
Slide orientation
- Refer to data sheet for slide orientation.
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