Category Human tissue array    Cat. No. CR    Product description Esophagus cancer    Data sheet CR1 CR1        Images   general_information    No. of samples 60    Text Current version: CR1 Esophagus cancer: 60 cores

Price : 5 slide for 600 €

General Information of the Tissue Array Slide :

Applications

• 􀁺 Immunohistochemistry
• 􀁺 In situ hybridization, RNA or DNA
• 􀁺 Fluorescent in situ hybridization (FISH)
• 􀁺 In situ PCR, RNA or DNA
• 􀁺 TUNEL for apoptosis (Recommended procedures in www.tissue-array.com -> protocol)

Storage and stability

• 􀁺 Individual slide is paraffin-coated and put in an air-tight pack with inert gas.
• 􀁺 Tissue array slides are shipped in room temperature.
• 􀁺 If the slides are stored in 4 degree, they are good up to one year.

How processed

• 􀁺 Tissues were initially fixed with formalin except for some of the animal tissues.
• 􀁺 Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
• 􀁺 Cleared by xylene, three changes for 1 hour each.
• 􀁺 Infiltrated with 60°C paraffin, three changes for 1 hour each
• 􀁺 Microtoming in 4 µm thickness and put on silane coating slide from Muto Pure Chemicals (Japan)

Before use

• 􀁺 Dry slides for 1 hour in a oven at 60 degree.
• 􀁺 Dewax slides in xylene for 4 minutes x 5 times
• 􀁺 Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
• 􀁺 Immerse slides in tap water for 5 minutes

Slide orientation

• 􀁺 In most of the slides with 59 or 60 cores, the orientation is as below unless indicated otherwise. #60 location is usually filled with carbon for orientation.