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Product Details: Epidermal Growth Factor (EGF) human
(RECOMBINANT)
Basic Information
|
|
| Product ID: |
GF-010-5 |
| Product Name: |
Epidermal Growth Factor (EGF) human
(RECOMBINANT) |
| Unit: |
100µg |
| Price: |
105 Euro |
| Availability: |
Available |
| Category: |
Growth Factors, Cytokines, Receptors
and their Antibodies |
| Subcategory: |
Growth Factors/Cytokines/Enzymes |
Product Details
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|
| Description: |
Human EGF is produced by genetically
engineered yeast, and purified by sequential
chromatography and reverse-phase HPLC. This polypeptide
is identical to gamma-urogastrone, or equivalent to
human EGF1-52.
|
| Biological Activity: |
The mitogenic activity of human EGF is
measured by the stimulation of 3H-thymidine
incorporation into human foreskin fibroblast cells. In
addition, EGF activity has been determined by a
receptor-binding assay (RBA) using A-431 cells.
|
| Formulation: |
Lyophilized powder |
| Purity: |
Over 97% pure by N-terminal amino acid
sequencing, amino acid composition, HPLC analysis, and
SDS gel electrophoresis. |
| Storage Conditions: |
Store at +4°C. Reconstitution is best
in water or buffer near neutral pH. |
| Stability: |
Several months at +4°C. In solution, at
pH 4-7, is stable for 2-3 months at -20°C. |
| Temperature: |
+4°C |
| References: |
1. Cohen, S. (1962) J, Biol. Chem.
237:1555
2. Gregory, H. (1985) J.Cell Science Supp.. 3:11
3.- George-Nascimento, C. et al. (1988) Biochemistry
27:797-802 |
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