DOPA
Home Up anti DKK1 Anti D D1R DAT dBMAL Dcytb Defensin 1-4 DLK1 ,ZOG ,Pref-1& FA1 DINE or ECEL1 or Xce DNA DNP1 DOPA D1-D5 & DAT DBH DRASIC

 

 

 

                         Dopamine Receptor, Dopamine Transporter & Dopamine Decarboxylase Antibodies

 


Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses. DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4). The two isoforms of D2R, D2 long (D2L) and short (D2S), are encoded by splice variants of a single gene and differ only by the presence of an additional 29 AA in the intracellular domain 3 of the D2 long form. It may play a role in the coupling of the receptor to G-proteins. All members of this family have similar structure and contain 7 putative transmembrane domains. A given cell or tissue may express more than one DR. Specific radioligands do not exist that can differentiate between these DR. Therefore, specific antibodies are needed to distinguish, localize, and document changes in DR levels in cells and tissues under various normal and pathological conditions.
ADI has made rabbit antibodies to specific to various DR using the DR-subtype specific amino acid sequences. These antibodies should prove to be useful in studying various DR using Western blotting and immunolocalization. Antibodies to Dopamine, Dopamine Transporter (DAT) and Dopamine Decarboxylase (DDC) and Dopamine Beta-hydroxylase (DBH) are also available.The control peptides, used for immunization, are also available to determine specificity of antibodies.

 

 Items Antigen peptide location   Ab
Host		  Expected Ab Crossreactivity  Neat Antisera
Cat #
(100 ul)		 Aff. Pure
Ab Cat #
(100 ug)		  * Control
Peptide Cat#
(100 ug)
D1 (Ab#1) R, CP, 13aa Rb M,R, H, P D1R11-S D1R11-A  D1R11-P
D1 (Ab#2) R, 9 aa, EC4 Rb M, R, H D1R12-S D1R12-A  D1R12-P
D2 (L/S) (Ab#1) R, 11 aa, EC1 Rb M, R, H, B, C, S D2R11-S D2R11-A D2R11-P
D2 (L) Ab # 2 R, 27 aa, CP3 Rb M, R, H, B D2R12-S D2R12-A D2R12-P
D2 (L/S) (Ab# 3) H, 27 aa, CP3 Rb M, R, H, B D2R13-S D2R13-A D2R13-P
D3 (Ab#1) H, 19 aa, EC3 Rb H D3R11-S D3R11-A D3R11-P
D3 (Ab#2) R, 19 aa, EC3 Rb M, R D3R12-S D3R12-A D3R12-P 
D4 (Ab#1)  M, 10 aa, EC3 Rb M, R, H D4R11-S D4R11-A D4R11-P
D4 (Ab#2) H, 17 aa, EC1 Rb H D4R12-S D4R12-A D4R12-P
DAT (Ab#1) R, 18 aa, I Rb M, R, H, D DAT11-S DAT11-A DAT11-P
DAT (Ab#2)  H, 20 aa, CT G M, R, H - DAT12-A DAT12-P
 

 DAT (Ab#3)

  

 H, 20 AA, ~CT

  

Rb

  

H, R, B

  

DAT13-S

  

DAT13-A

  

DAT13-P
DDC

(Ab # 1)

 H, 17 aa, NT M, Mono M, R, H, B, D   DDC11-M  

DDC (Ab # 2)

H, 16 aa, ~NT

Rb, poly

M, R, H, B, D

  

DDC12-A

DDC12-P
 

 DBH (ab # 1)

  

 B

 M, Mono M, R, H, B  

DBH11-S

DBH11-M

  
 

 DBH (ab # 2)

  

H, ~18 aa, ~NT

  

Rb

  

H, M, R

  

.

  

DBH12-A (50 ug)

  

 DBPH12-P
 

 Dopamine (ab # 1)

  

 Dopamine

  

 Rb

  All species  

DOP11-S

    

Dopamine (Ab #2)

Dopamine

m, mono

All Species

  

DOP12-M

  
 Control Rabbit IgG  For use in ELISA, Western, Immunohisto. 20009-1 (1 mg)  



M= Mouse; R=Rat; H=Human; Ha=Hamster; Rb=Rabbit; B=Bovine; C=Chicken; D=DOG; CT= near C-terminus; NT=near N-terminus; Internal=Middle of protein; CL=Cytoplasmic loop; EC=Extracellular domain; IC=Intracellular domain;

"Neat Antisera" are the unpurified antiserum and it is suitable for ELISA and Western.
"Affinity pure" antibodies have been over the antigen-affinity column and recommended for immunohistochemical applications.
"Control peptides" can not be used for Western as they are very short peptides. They are intended for ELISA or antibody competition studies.

All Products are for in vitro research use only.

 

  

List of publications using ADI's Antibodies to various Dopamine Receptor and Related Products

DAT Lotharius J 2002 Effect of Mutant -Synuclein on Dopamine Homeostasis in a New Human Mesencephalic Cell Line JBC 277, 38884-38894 WB IHC human Mesencephalic Cell Line.

 

D1R11 Banday AA/Hussain T 2003 Hypertension, Apr 2003; 10000069. Dopamine-Mediated Inhibition of Renal Na,K-ATPase Is Reduced by Insulin WB, rat kidney.

D1R11 Umrani DN/Hussain T 2002 Hypertension 40: 880 - 885 Rosiglitazone Treatment Restores Renal Dopamine Receptor Function in Obese Zucker Rats WB, rat.

D1R11 Beheray, Sucheta 2000 Kidney Intl. 58, 712 Diminished natriuretic response to dopamine in old rats is due to an impaired D1-like receptor-signaling pathway WB.

 

D2R Kamiya T 2003 Oligomerization of adenosine A2A and dopamine D2 receptors in living cells Biophysical Research Communications, Volume 306, Issue 2, 27 June 2003, Pages 544-549. WB, IHCIF, IP Human cells cDNA.

D2R Farooqui SM et al 1992 Biochem Biophys Res Commun. 1992 Apr 30;184(2):661-7. Production and characterization of a monoclonal antibody to dopamine D2 receptor: comparison with a polyclonal antibody to a different epitope WB, IHC.

D2R Sakata M 1992 Biochem Biophys Res Commun. 575, 309-314 Post-transcriptional regulation of loss of rat striatal D2 dopamine receptor during agina WB, 110 kda rat brain.

D2R Farooqui SM 1991 J. Neurochem., 57, 1363-1369 Antibodies against synthetic peptide predicted from the nucleotide sequence of D2 R recognize native D2R protein in rat striatum WB 120, 90, 85, 30 ka rat brain.

D2R Brock JY 1992 Brain Res. 578, 244-250 Localization of D2R in rat brain using polyclonal antibody
IHC rat brain 4% PF.

D2R Farooqui SM 1992 Life Sci. 51, 1509-1516 An antibody to D2R inhibits dopamine antagonist and agonist binding to D2R cDNA transfected mosue fibrobalst cells ligand binding and flow cytometry.

 

D3R Chu, Eugenia 2000 J. Pharmacol. Exp. Ther. 2000 293: 710-716 Mechanisms and Sites of Ocular Action of 7-Hydroxy-2-dipropylaminotetralin: A Dopamine3 Receptor Agonist IHC, rabbit eyes.

 

D3R12 Zeng C 2002 Hypertension, 41, 724-729 Angiotensin II Regulation of AT1 and D3 Dopamine Receptors in Renal Proximal Tubule Cells of SHR WB IP D3R from SC as well rat RPY cells

D3R12 Ladines, Cecilia A. 2001 Am J Physiol Regulatory Integrative Comp Physiol 2001 281: R1071-R1078 Impaired renal D1-like and D2-like dopamine receptor interaction in the spontaneously hypertensive rat WB, renal cortical memb 45/90kda.

 

Notes: Antibodies usage is indicated in the following techniques: WB=Western Blot ; IHC-Immunohistochmistry; IP=Immunoprecipition; Flow=Flow cytometry; Rev. 30204