DNA Walking

DNA Walking SpeedUp^TM kit provides information about
unknown sequences adjacent to upstream or
downstream known sequences.

Previous methods commonly used for this purpose include inverse PCR (IPCR), ligation-mediated PCR (LM-PCR) and randomly primed PCR (RP-PCR).

Although these methods have been used efficiently and have proven to be effective,
they are still hampered by laborious steps such as restriction enzyme digestion,
adaptor ligation, and purification procedures. Moreover, arbitrary pimers in
RP-PCR often result in non-specific products due to non-specific annealing.

These drawbacks of existing genomic walking methods can be overcome by
employing Seegene's proprietary ACP^TM (Annealing Control Primer)

Seegene developed DNA walking technology by incorporating ACP^TM (Annealing Control Primer) and DSO^TM (Dual Specificity Oligonucleotide) technologies, which maximize the specificities of short primers and target specific primers, respectively, and furnishes optimized data about unknown sequences.

Note! DNA Walking^TM kit II, which provides widen application and higher success ratio
than DNA Walking^TM kit I, has been recently released.

The simplest method
: This method requires NO complex process,
NO restriction enzyme digestion, NO immobilization, and NO complex PCR.

The fastest method
: Your target products can be obtained within 1 day, which is much faster than
currently available methods.

The most reliable method
: Only target products are able to be obtained.

Upgrade!! DNA Walking^TM kit II.

1. Widen Application !!
Kit allows various vector mediated transgene integration site identification
as well as unknown sequence identification

2. Higher Success Ratio !!
Kit is highly optimized for unknown sequence identification

3. Longer PCR Product !!
Kit generates long PCR products that you can easily analyze




Cat. No	Product	Size


K1501	DNA Walking SpeedUp¢â Kit I	10 rxns

K1502	DNA Walking SpeedUp¢â Kit I	25 rxns

K1503	DNA Walking SpeedUp¢â Kit II	15 rxns