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| GENTAUR Belgium : + 32 2 7325688 France : 01 43250150 Italy : 02 36006593 Germany : +49 241 6085 13140 | |
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For USA call : (800) 631-9384 CYTOCHROME P450 RESEARCH PRODUCTS
See our NATIVE ISOLATES
of the following-please click for more details: CYP2A6, CYP2C9, P450
reductase & cytochrome b5. Certain research has indicated that there may be some
discrepancies between recombinant and native isolates. These native enzymes will
allow you an opportunity to check your system for both types. A select group of
recombinant enzymes are also now available :
Anti-Cytochrome P450 Antibodies (rabbit polyclonal). P450 antibodies give 90% inhibition of prototypic reactions catalyzed by the corresponding Ag in intact liver microsomes. "All P450 abs except the CYP2C IgGs react specifically with their respective antigens on western blots. CYP2C9 and CYP2C19 abs cross-react with other CYP2C proteins in human liver (unless marked as specific). Available as purified rabbit IgG fractions (lyophilized).
RDI-CYP2A6abr 350.00/1mg RDI-CYP2C8abr currently sold out RDI-CYP2C9abr 350.00/mg RDI-CYP2E1abr 350.00/1mg RDI-CYP2C19Mabr 800.00/1mg (specially backabsorbed to reduce reactivity with CYP2C8 and CYP2C9) RDI-CYP2C19abr 550.00/1mg (more sensitive for CYP2C19 but also sees CYP2C8 and CYP2C9 in full size blots RDI-CYP3A4abr 350.00/mg (also sees 3A5 & 3A7) RDI-CYP3A4Mabr 450.00/mg (specific for 3A4) RDI-CYP2D6abrX 350.00/vial (at least 10 western blots) RDI-CYP4A11abr 350.00/mg RDI-CYP4F2abr 375.00/mg RDI-CYP2C10abrX 350.00/vial (at least 10 western blots) RDI-NORMCTRL (non immune control serum 100.00/mg)
RDI-P450REDabr 350.00/0.1ml anti-cytochrome P450 reductase RDI-P450SCCabr 350.00/0.1ml anti-cytochrome P450 side chain cleavage enzyme RDI-CYP1A1abr 350.00/0.1ml anti-cytochrome P450 1A1 RDI-CYP1A2abr 350.00/0.1ml anti-cytochrome P450 1A2 RDI-CYP2B12abr 350.00/0.1ml anti-RAT cytochrome P450 2B1 & 2B2 RDI-RTCYP2C11abm 350.00/vial mouse anti-rat CYP450 2C11 RDI-RTCYP2C11abr 350.00/0.1ml anti-rat CYP2C11 RDI-CYP2A6B6abr 350.00/0.1ml anti-cytochrome P450 2B6 & 2A6 -also available(anti-human CYP1A1, CYP3A2, & CYP3A5) -see also monoclonals to P450's see also antibodies to rat: CYP1A1, CYP1A2, CYP2B1/2, CYP2C12, CYP2D1, CYP2D4, CYP2E1, CYP3A2, CYP4A1/2/3, CYP450 reductase, CYP 450 side chain cleavage and other against human
CYTOCHROME P450 Research Products Suggested Protocol for Standard Reconstitution recombinant Cytochrome P450's: -ref: Dieter, H. and Johnson, E.F. (1982) J. Biol. Chem. 257:9315-9323. Components: Cytochrome P450 Cytochrome P450 Reductase Lipid (L-alpha-phosphatidylcholine dilauroyl ( C 12:0) Sigma Chemical cat#P-1263 NADPH 50mM Potassium Phosphate Buffer pH 7.4 (KPi buffer) pH by mixing monobasic and dibasic KPi
Preparation of lipid: A stock solution (3mg/ml) is prepared fresh in KPi buffer. Prior to use, the lipid solution must be dispersed by four 5 second pulses at 20% power using a Branson 200 series sonifier (or similar device) fitted with a microprobe.
Reconstitution: To assess substrate metabolism, combine 10 pmole cytochrome P450 (example: 10ul of a 1 nmole/ml solution) with 0.3 units cytochrome P450 reductase (example 10ul of a 30 unit/ml solution) and 30ug freshly prepared lipid (example 10ul of a 3 mg/ml solution) and incubate on ice for 10 minutes. To this add the substrate and kPi buffer to a final volume of 980ul and mix (in most cases, the final concentration of the substrate solvent should not exceed 1%). Incubate this mixture for 3 minutes at 37 DEG C. The reaction is initiated by the addition of NADPH to a final concentration of 1mM (20ul of a 50 mM stock solution).
Reductase Dilution buffer: 10mM KPi, pH 7.4, 20% glycerol, 0.1Mm EDTA.
Cytochrome P450's and select substrates Cytochrome P450 2C8: taxol, retinoic acid, benzo{a}pyrene, arachidonic acid, warfarin Cytochrome P450 2C9: tolbutamide, phenytoin, arachidonic acid, intienilic acid, warfarin Cytochrome P450 2C18: warfarin Cytochrome P450 2C19: S-mephenytoin, diazepam, tolbutamide, progesterone, warfarin Cytochrome P450 2D6: debrisoquine, sparteine, bufuraol, warfarin
CYTOCHROME P450 INHIBITORS Below are some of the various compounds with known inhibition activity of the cytochrome P450 enzyme. Please contact us for additional compounds. All materials are for in vitro research use only-not for use in diagnostics-not for use in or on humans or animals . CAT# Description Price RDI-R41400 R 41400 A.S. Ketoconazole 99+% 50mg 250.00 (inhibits Cp450c17) Kuhn-Velten et al, Biochem. Pharmacol. 44 (12), 2371 (1992). RDI-R51211 R 51211 A.S. Itraconazole 99+% 50mg 250.00 RDI-R18134 R 18134 A.S. Miconazole 99+% 50mg 250.00 RDI-R14950 R 14950 A.S. Miconazole Nitrate 99+% 50mg 250.00 |
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