DESCRIPTION
This homogeneous assay involves simply adding a single reagent, the
CellQuanti-Blue reagent, to the cell culture and measuring the fluorescence
intensity (excitation wavelength = 530 - 570 nm, emission wavelength = 590 -
620 nm) after an incubation step. The CellQuanti-Blue reagent, like other
resazurin-based assays such as the Alamar Blue reagent, utilizes the redox
dye resazurin which is not fluorescent, but upon reduction by metabolically
active cells is converted into a highly fluorescent product (resorufin).
Living cells can readily reduce this non-toxic reagent and the resulting
increase in fluorescence intensity can be conveniently monitored using a
fluorescence spectrophotometer or plate reader. Nonviable cells have no
metabolic capacity and, thus, will not reduce the dye. Therefore, the
fluorescence intensity observed in this assay is a true measure of the
viable cells.
The CellQuanti-Blue reagent has been optimized for maximum sensitivity,
reproducibility and long shelf-life. The homogeneous cell-based assay can be
performed in multi-well plates. The reagent is compatible with all culture
media and with all liquid handling systems for high-throughput screening
applications in 96-well and 384-well plates. Applications include cell
proliferation, cytotoxicity and apoptosis.
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