Cachectic Factor
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Product Name Human Cachectic Factor (CF) Antibodies
 Cat. # CF11-S Rabbit Anti-Human CF antiserum, neat serum, SIZE: 100 ul,
 Cat. # CF11-P Human CF Control Peptide , SIZE: 100 ug/100 ul,
 Introduction Cancer cachexia is a syndrome of progressive wasting which is believed to be mediated by tumor necrosis factor-alpha, interleukins 1 and 6, and interferon-gamma, and leukemia-inhibitory factor. However, the levels of these putative agents do not correlate with cachexia. Recently, a new circulating tumor factor, termed cachectic factor (CF), has been isolated and characterized from the urine of mice transplanted with adenocarcinoma MAC16 (1). Purified CF, a proteoglycan of relative mol. Wt of ~24 kDa, produces cachexia in vivo by inducing catabolism of skeletal muscle. CF was also found in urine of cachectic cancer patients. It is not found in normal urine, patient with weight loss due to trauma, and cancer patient with no weight loss (1).

CF appears to be ~24 K on SDS-gels. It is a sulfated glycoprotein. The amino acid analyses was determined as: YSP EAA SAP GSG DPS HEA (S) (A). An antibody raised to the CF also recognized a 69 kDa band with the same amino acid sequence as the 24 K material.
Source of Antigen and Antibodies An 18 AA Peptide (designated CF11-P; control peptide) corresponding to the sequence of CF (YSP EAA SAP GSG DPS HEA) was selected for antibody production (1). The peptide was coupled to KLH, and antibodies generated in rabbits.
Form & Storage Control peptide Solution is provided in PBS, pH 7.4 at 1 mg/ml (100 ug/100 ul). Antiserum is supplied as neat serum (100 ul soln or lyophilized). Affinity pure antibodies were purified over the peptide-Sepharose column and supplied as 1 mg/ml soln in PBS, pH 7.4 and 0.1% BSA as stabilizer (100 ul in solution or Lyophilized). The peptides and antibodies also contain 0.1% sodium azide as preservative. Lyophilized products should be reconstituted in 100 ul water and gently mixed for 15 min at room temp. All peptide/antibody received in solution or reconstituted from lyophilized vials should be stored frozen at -20oC or below in suitable aliquots. It is not recommended to store diluted solutions. Avoid freeze and thaw.
Recommended Usage
 Western Blotting (1:500-2K for neat serum) using ECL technique).
 ELISA Control peptide can be used to coat ELISA plates at 1 ug/ml and detected with antibodies (1:10-50K for neat serum and 0.5-1 ug/ml for affinity pure).
 Histochemistry & Immunofluorescence :Not tested. We recommend the use of affinity purified antibody at 1:200-1:500 in paraformaldehyde fixed sections of tissues.
Specificity & Cross-reactivity The 18 AA human CF is unique to CF. It has some sequence homology with streptococcal preadsorbing antigen involved in pathogenesis of acute post-streptococcal glomerulonephritis (2). No significant sequence homology is detected with other proteins. Antibody cross-reactivity in various species has not been studied. The CF11-P control peptide is available to confirm specificity of antibodies.
 References 1. Todorov P et al (1996) Nature 379, 739-742; Todorov PT et al (1997) J. Biol. Chem. 272, 12279; Cariuk P et al (1997) Br. J. Cancer 76, 606-613; Cancer Res. (1995) 55, 1458-1563.
2. Yoshizawa N et al (1992) J Immunol. 148, 3110-3116..
   *This product is for In vitro research use only.