|
|
|
|
Bombesin Receptor 3 (BRS-3) Antibodies
Bombesin (BN) is a tetradecapeptide (Pyro-ENR LGN
QWA VGH LM-amide) that was originally isolated from frog skin. Several
BN-related peptides have been isolated from amphibians and classified into three
subfamilies: Bombesin, ranatensin, and phyllolitorin based upon the last three
C-terminal residues. Two BN-like peptides have been identified in mammals: NMB
(neuromedin B) in the ranatensin family and GRP (gastrin-releasing peptide) in
the BN-family. BN-family of G-protein coupled receptors include at least four
receptor subtypes: The GRP-preferring receptors (GRP-R or bb2), the
NMB-preferring receptors (NMB-R or bb1) and Bombesin receptor subtype 3 (BRS-3
or bb4). These receptors share approx. 50% amino acid homology and bind
bombesin. However, BRS-3 has much lower affinity for BN than GRP-R and NMB-R.
Mammalian BN-like peptides are widely distributed in the brain and
gastrointestinal tract, where they modulate smooth-muscle contraction, exocrine
and endocrine activities, metabolism and behavior. Most recently, BRS-3
deficient mice have been shown to develop mild obesity, associated with
hypertension, and impairment of glucose metabolism, reduced metabolic rate,
increased feeding efficiency and subsequent hyperphagia. Human BRS-3 gene
(chromosome x) encodes a 399 aa protein with seven transmembrane domains. The
NH2 and COOH-termini are predicted to be extracellular and cytoplasmic,
respectively. ADI has produced rabbit-anti BRS-3 using BRS-3 specific peptide
sequences from the C-terminal cytoplasmic domain. The appropriate control
immunogenic peptide is also available to confirm specificity of antibodies. |
|
|