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Specification sheet |
Recombinant Human BMP-2
|
| Catalog
No:PC105-1 |
2
micrograms |
180€ |
| Catalog
No:PC105-2 |
5
micrograms |
225€ |
| Catalog
No:PC105-3 |
10
micrograms |
275€ |
| Catalog
No:PC105-4 |
25
micrograms |
560€ |
| Catalog
No:PC105-5 |
100
micrograms |
CALL/EMAIL |
| Catalog
No:PC105-6 |
1 mg |
CALL/EMAIL |
| Source |
E.
Coli |
| Formulation |
The sterile filtered solution was lyophilized from 10 mM
Sodium Citrate, pH 3.5. |
| Reconstitution |
Centrifuge briefly to
bring the protein down from the top of the vial. Reconstitute in water
containing BSA (50 μg of BSA per 1 μg of protein) to a concentration of
0.1-1.0 mg/mL. This solution can then be diluted into other buffers and
stored at 4 degree Celsius for 1 week or stored at -20 degree Celsius for
future use. Avoid repeated freeze-thaw cycles. |
| Storage |
The lyophilized
protein is stable for a few weeks at room temperature, but best stored at
-20 degree Celsius. Reconstituted BMP-2 should be stored in working aliquots
at -20 degree Celsius. |
| Purity |
greater than 98% by
SDS-PAGE. |
| Biological
Activity |
The ED50 as
determined by the cytolysis of MC3T3-E1 cells is less than 50 ng/mL. |
| Description |
BMPs (bone
morphogenetic proteins) belong to the TGF-beta superfamily of structurally
related signaling proteins. Members of this superfamily are widely
represented throughout the animal kingdom and have been implicated in a
variety of developmental processes. Proteins of the TGF-beta superfamily are
disulfide-linked dimmers composed of two 12-15 kDa polypeptide chains. As
implied by their name, BMPs initiate promote and regulate bone development,
growth, remodeling and repair. However, it is now clear that in addition to
their role in bone and cartilage morphogenesis, BMPs are also involved in
prenatal development and postnatal growth of eye, heart, kidney, skin and
other tissues.
In addition to its osteogenic activity, BMP-2 plays an important role in
cardiac morphogenesis. It is also expressed in a variety of tissues such as
lung, spleen, brain, liver, prostate ovary and small intestine. Recombinant
human BMP-2 is a 26 kDa homodimeric protein consisting of two 115 amino acid
polypeptide chains.
|
| Endotoxin |
Endotoxin level les
than F0.1 ng/μg. |
| Country of
Origin |
USA |
|
