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Cat. # NKCC11-P, Rat NKCC1 Control Peptide, SIZE: 100 ug Chloride is a critical component of all living cells. It is also the single most dominant diffusible anion inside of most cells - the others are mostly impermeable organic anions. The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane. It includes the thiazide-sensitive Na+-Cl-cotransporters (NCC or TSC), the loop diuretics-sensitive Na+-K+-Cl- (NKCC) cotransporters (NKCC1/CCC1/BSC2 and NKCC2/CCC2/BSC1), and the K+-Cl--cotransporters (KCC1-4). These co-transporters share a common predicted membrane topology, with 12 TM domains (~500 aa), and long hydrophilic, intracellular N-and C-termini containing regulatory phosphorylation sites. NKCC transport Na, K, and Cl ions into and out of a wide variety of epithelial and non-epithelial cells. The transport process is characterized by electroneutrality (almost always with stoichiometry of 1Na:1K:2Cl) and inhibition by the loop diuretic bumetanide, benzametanide, and furosemide.
NKCC1/BSC2 (human 1212 aa, rat 1203 aa, and mouse 1205 aa; calculated mol. wt of ~115 kDa; actual size is ~145kDa) is strongly expressed in the kidney and many other tissues. In mouse NKCC1 is detected in basolateral membrane of IMCD and in mesangial cells in the glomerulus and basolateral membrane of acinar cells in rat submandubular gland. In the brain, NKCC1 mRNA is most prominent in choroid plexus. Source of Antigen and Antibodies Antigen: 22aa peptide of Rat NKCC; Designated (NKCC11-P). Recommended Usage Western Blotting (1:1K-5K for neat serum and 1-10 ug/ml for affinity pure using Chemiluminescence technique). ELISA (1:10K-1:100K; using 50-100 ng of control peptide/well). Histochemistry & Immunofluorescence: Not tested. We recommend the use of affinity purified antibody at 2-20 ug/ml. Specificity & Cross-reactivity The 22 AA rat NKCC11-P control peptide is 95% conserved in mouse, 86% in human, and 82% in boivne NKCC1. No significant sequence homology is detected with other CCC. Antibody cross-reactivity in various species has not been studied. The NKCC11-P control peptide, because of its small size (<3 kDa), is not suitable for Western. It should be used in ELISA, dot blot or to neutralize antibody and confirm specificity of antibody. References: (1) Moore-Hoon,M.L. et al (1998) Biochim. Biophys. Acta 1373, 261; Delpire,E., et al (1994) J. Biol. Chem. 269, 25677; Payne,J.A., et al (1995) J. Biol. Chem. 270, 17977; Isering P et al (1997) J Biol. Chem. 272, 24556; Mount DB et al (1998) J. Exptl. Biol. 201, 2091-2102 (review). |
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