The beta 3-adrenergic receptor (B3AR) is a member of the super-family of G protein-coupled receptors that are characterized by seven putative transmembrane helices connected by hydrophilic loops. The mechanism by which the activated beta ARs transmit the signals across the plasma membrane involves the stimulation of Gs, which in turn activates adenyl cyclase, yielding the second messenger cAMP. (B3AR) is present primarily in adipocytes The beta 3-adrenoceptor plays a significant role in the control of lipolysis and thermogenesis in the brown adipose tissue of rodents and humans. In human beta 3-adrenoceptor, a Trp to Arg replacement has recently been discovered. This change which occurs at position 64, in the first coding exon, has been correlated with increased weight gain, difficulty in losing weight, insulin resistance syndrome, and worsened diabetic situation. Higher percentages of this mutation are observed in Pima Indians (over 30%) and Japanese (20%). Thus, chronic stimulation of the beta 3-adrenergic receptor by highly selective B3AR agonist CL316,243, induces ectopic expression of UCP1 in adipose tissues and skeletal muscle, which may contribute to the potent anti-obesity effect of the beta 3-adrenergic agonist. Human B3AR is a 408 AA membrane protein (rat/mouse, 400 AA). The N-terminus is predicted to be extracellular, while the C-terminus intracellular. ADI has produced rabbit-antibodies using mouse and human B3AR specific peptide sequences. The appropriate control immunogenic peptides are also available to confirm specificity of antibodies.

~CT=near C-terminus; m=mouse, r=rat; h=human; c=canine; b=bovine.

* Expected antibody crossreactivity information is mostly based upon high (>70%) sequence conservation of antigenic/control peptides in various species. When antibody crossreactivity has actually been experimentally confirmed in various species, it will be mentioned in the appropriate data sheets.

"Neat Antisera" are the unpurified antiserum and it is suitable for ELISA and Western.
"Affinity pure" antibodies have been over the antigen-affinity column and recommended for immunohistochemical applications.
"Control peptides" can not be used for Western as they are very short peptides. They are intended for ELISA or antibody competition studies.

List of Publications using ADI's antibodies for Andrenergic Receptors:
 

• Cirino G 2003 PNAS, Apr 2003; 100: 5531 - 5536 Involvement of 3-adrenergic receptor activation via cyclic GMP- but not NO-dependent mechanisms in human corpus cavernosum function WB, IHC also from SC-compared human penis etc

• Jost P 2002 Am J Physiol Endocrinol Metab : 283, E146-E153 Atypical -adrenergic effects on insulin signaling and action in 3-adrenoceptor-deficient brown adipocytes WB, IP mouse adipocyte

• Cheng H-J et al 2001 Circ. Res. 89: 599-606 Upregulation of Functional ß3-Adrenergic Receptor in the Failing Canine Myocardium WB, canine lV myocardium