Anti-NSF Polyclonal Antibody

CATALOG #:                                                                          3541-100

AMOUNT:                                                                               100 mg

HOST:                                                                                     Rabbit

 FORMULATION:

100 mg (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.

 SPECIES REACTIVITY:                                          mouse, rat

 STORAGE CONDITIONS:      

Store at -20^oC. For long term storage, aliquot and freeze at –70^oC. Avoid repeated freeze/defrost cycles.

 BACKGROUND:

Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimidesensitive factor), SNAP 25 (synaptosomal-associated protein of 25kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis by competing with SNAP 25 and syntaxins for VAMP binding.

 SPECIFICITY:        

The antibody recognizes 85 kDa NSF of mouse, and rat origins. Reactivity to other species has not been tested. 

 APPLICATION:

The antibody can be used in Western blotting (1-2 mg/ml), immunoprecipitation (5-15 mg/ml) and immunohistochemistry (10-20 mg/ml). However, the optimal concentrations should be determined individually.

 USAGE:                   For Research Use Only. Not to be used in human.

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