Anti-MAGE(IN) CATALOG No.
28004 299 Euro /100ul
BACKGROUND:
The melanoma-associated antigen (MAGE) gene family includes at least 17
related genes, namely MAGE-A1 to A12, MAGE-B1 to B4 and MAGE-C1 (1) The MAGE
genes are expressed by tumors of various histological types, but they are
silent in normal cells, with the exception of male germ-line cells. Thus the
antigens encoded by MAGE-A, -B, -C genes should be strictly tumor specific.
Because the MAGE antigens are shared by many tumors and on account of their
strict tumor specificity, they are of particular interest for cancer
immunotherapy (2).
SOURCE:
Rabbit anti-MAGE polyclonal antibody was raised against a synthetic peptide
corresponding to amino acids 245-259 of human MAGE-1(QEKYLEYRQVPDSDP). There
are two amino acids different from mouse.
REACTIVITY:
This epitope affinity purified rabbit polyclonal antibody is specific for
MAGE-1. The antibodies were evaluated for specificity by
immunohistostaining. A positive staining is observed in human Melanoma.
APPLICATION:
The working concentrations for specific applications should be determined by
the investigator. The results might be affected by several factors,
including secondary antibody
affinity, antigen concentration, sensitivity of detection method,
temperature and length of incubations.
ELISA: 0.1-1.0 µg/ml.
Western blot: 0.5 to 2 µg/ml.
IP: 3.0-5.0 µg/extract from 107 cells.
IHC: 1:100
STORAGE:
This polyclonal antibody is supplied as an epitope affinity purified rabbit
IgG, 100 µg in 200 µl of phosphate buffered saline (pH 7.4) containing 0.02%
sodium azide. Store at 2-8 °C for up to one month. Store at –20 oC for
long-term storage. Avoid repeated freezing and thawing.
REFERENCES:
1. Gillespie AM et al (1999) Cancer Treat. Rev. 25, 219-227.
2. Chames P et al (2000) Proc. Natl. Acad. Sci. USA 97(14), 7969-7974.
Human melanoma stained with anti-MAGE (Cat# 28004) by IHC Histain Kit-AEC
(Cat# 29702). The formalin-fixed tissues was boiled in 10 mM citrate buffer,
pH 6.0 for 10 min, followed by cooling @ RT for 20 min, then probed at a
dilution 1:100 for 10 min at room temperature.
