Anti CML NF-1G



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Code No. 68-KH024 50ug price: 631 Euro / 695 USD

Advanced Glycation End Products AGE

Anti CML Monoclonal Antibody

(Clone No. NF-1G)

Reaction of protein amino groups with glucose leads, through the early products such as a Schiff base and

Amadori rearrangement products, to the formation of advanced glycation end products (AGE). Recent

immunological studies using anti-AGE antibody (6D12) demonstrated the presence of AGE-modified proteins

in several human tissues: (1) human lens (nondiabetic and noncataractous), (2) renal proximal tubules in

patients with diabetic nephropathy and chronic renal failure, (3) diabetic retina, (4) peripheral nerves of

diabetic neuropathy, (5) atherosclerotic lesions of arterial walls, (6)ﾀ2-microglobulin forming amyloid fibrils in

patients with hemodialysis -related amyloidosis, (7) senile plaques of patients with Alzheimer’s disease, (8) the

peritoneum of CAPD patients, (9) skin elastin in actinic elastosis, and (10) ceriod/lipofuscin deposits. These

results suggest a potential role of AGE-modification in normal aging as well as age-enhanced disease

processes.

This antibody named as 6D12 has been used to demonstrate AGE-modified proteins in these human tissues,

indicating potential usefulness of this antibody for histochemical identification and biochemical quantification

of AGE-modified proteins.

N'-(carboxymethyl)lysine (CML) is a major antigenic AGE structure in vivo and is known to be generated

from Oxidative cleavage of Amadori product. In addition to amadori product, CML formation also takes place

through glyoxal, which is generated from the autoxidation of glucose and unsaturated fatty acids. NF1G is

monoclonal antibody specific for CML and useful for immunohistochemical staining to demonstrate the

localization of CML in some pathological tissues.

Format : Mouse monoclonal antibody 0.25 mg/mL

Package Size : 50µg (200µL/vial)

Buffer : Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat

Storage Store below –20. Once thawed, store at 4. Repeated freeze-thaw cycles should be avoided.

Clone No. : NF-1G

Subclass : IgG2a

Purification method :

The splenic lymphocytes from BALB/c mouse, immunized with CML-HSA

were fused to myeloma P3U1 cells. The cell line (NF1G) with positive reaction

was grown in ascitic fluid of BALB/c mouse, from which the antibody was

purified by Protein G affinity chromatography.

Working dilution for immunohistochemistry : 5-10µg/mL; for ELISA: 0.1-1.0µg/mL

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