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| GENTAUR • Belgium : + 32 2 7325688 • France : 01 43250150 • Italy : 02 36006593 • Germany : +49 241 6085 13140 | |
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For Japan Tel:+81-96-212-3664 Code No. 68-KH011 50ug price: 531 Euro / 695 USD Advanced Glycation End Products AGE Anti CML Monoclonal Antibody(Clone No. CMS10) Reaction of protein amino groups with glucose leads, through the early products such as a Schiff base and Amadori rearrangement products, to the formation of advanced glycation end products (AGE). Recent immunological studies using anti-AGE antibody (6D12) demonstrated the presence of AGE-modified proteins in several human tissues: ( 1) human lens (nondiabetic and noncataractous), (2) renal proximal tubules inpatients with diabetic nephropathy and chronic renal failure, (3) diabetic retina, (4) peripheral nerves of diabetic neuropathy, (5) atherosclerotic lesions of arterial walls, (6)ﾀ2-microglobulin forming amyloid fibrils in patients with hemodialysis -related amyloidosis, ( 7) senile plaques of patients with Alzheimer’s disease, (8)the peritoneum of CAPD patients, (9) skin elastin in actinic elastosis, and (10) ceriod/lipofuscin deposits. These results suggest a potential role of AGE-modification in normal aging as well as age-enhanced disease processes. This antibody named as 6D12 has been used to demonstrate AGE-modified proteins in these human tissues, indicating potential usefulness of this antibody for histochemical identification and biochemical quantification of AGE-modified proteins.
N'-(carboxymethyl)lysine (CML) was a major AGE structure identified by Banes et al. in 1989. Oxidative cleavage of Amadori products is considered as a major route to CML formation in vivo. Banes also revealed that CML was directly formed from the reaction between lipidoxidative products and Lysine residue. Thus, CML could become a marker of oxidative stress and long term damage to protein in aging, atherosclerosis, and diabetes.
Format : Mouse monoclonal antibody 0.25 mg/mL Package Size : 50 µg (200µL/vial)Buffer : Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat Storage Store below –20. Once thawed, store at 4. Repeated freeze-thaw cycles should be avoided. Clone No. : CMS-10 Subclass : IgG 1Purification method : The splenic lymphocytes from BALB/c mouse, immunized with CML-KLH were fused to myeloma P3U1 cells. The cell line (CMS-10) with positive reaction was grown in ascitic fluid of BALB/c mouse, from which the antibody was purified by Protein G affinity chromatography. Working dilution for immunohistochemistry : 5-10 µg/mL; for ELISA: 0.1-1.0µg/mL |
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