Anti CEL KNH-30
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Code No. 68-KH025                                           price: 531 Euro/695USD 366

Advanced Glycation End Products AGE

Anti CEL Monoclonal Antibody

(Clone No. KNH-30)

Reaction of protein amino groups with glucose leads, through the early products such as a Schiff base and

Amadori rearrangement products, to the formation of advanced glycation end products (AGE). Recent

immunological studies using anti-AGE antibody (6D12) demonstrated the presence of AGE-modified proteins

in several human tissues: (1) human lens (nondiabetic and noncataractous), (2) renal proximal tubules in

patients with diabetic nephropathy and chronic renal failure, (3) diabetic retina, (4) peripheral nerves of

diabetic neuropathy, (5) atherosclerotic lesions of arterial walls, (6)ﾀ2-microglobulin forming amyloid fibrils in

patients with hemodialysis-related amyloidosis, (7) senile plaques of patients with Alzheimer’s disease, (8) the

peritoneum of CAPD patients, (9) skin elastin in actinic elastosis, and (10) ceriod/lipofuscin deposits. These

results suggest a potential role of AGE-modification in normal aging as well as age-enhanced disease

processes.

This antibody named as 6D12 has been used to demonstrate AGE-modified proteins in these human tissues,

indicating potential usefulness of this antibody for histochemical identification and biochemical quantification

of AGE-modified proteins.

 

CEL is known to generate from protein modification by methylglyoxal . Mclellan et al. demonstrated that

plasma methylglyoxal, which is believed to be generate from Embden-Meyerhof and polyol pathways,

concentrations in insulin-dependent diabetic patients were about 7-times higher than those of normal

individuals. For examples, CEL was identified in human lens proteins at a concentration similar to that of CML

and its accumulation increased with age like CML, indicating that CEL may play an important marker for aging

and age-dependent disease such as diabetic complications.

 

Format :             Mouse monoclonal antibody 0.25 mg/mL

Package Size :    50µg (200µL/vial)

Buffer :               Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat

Storage Store below –20. Once thawed, store at 4. Repeated freeze-thaw cycles should be avoided.

Clone No. :         KNH-30

Subclass :            IgG1

Purification method :

                            The splenic lymphocytes from BALB/c mouse, immunized with CEL-BSA      

                            were fused to myeloma P3U1 cells. The cell line (KNH-30) with positive

                            reaction was grown in ascitic fluid of BALB/c mouse, from which the antibody

                            was purified by Protein G affinity chromatography.

Working dilution for immunohistochemistry : 5-10µg/mL; for ELISA: 0.1-1.0µg/mL