A 22 amino acid sequence nera the NH2-terminus of rat AR gene (1, 2) was used to generate anti-AR antibodies. A cysteine has been added at the C-terminus for coupling to KLH. The peptide was coupled to KLH and antibodies generated in rabbits. Antibody has been affinity purified using control peptide-Sepharose.

Form & Storage

Control peptide Solution is provided in PBS, pH 7.4 at 1 mg/ml (100 ug/100 ul). Antiserum is supplied as neat serum (100 ul soln or lyophilized). Affinity pure antibodies were purified over the peptide-Sepharose column and supplied as 1 mg/ml soln in PBS, pH 7.4 and 0.1% BSA as stabilizer (100 ul in solution or Lyophilized). The peptides and antibodies also contain 0.02% sodium merthiolate as preservative.

Lyophilized products should be reconstituted in 100 ul water and gently mixed for 15 min at room temp. All peptide/antibody received in solution or reconstituted from Lyophilized vials should be stored frozen at -20oC or below in suitable aliquots. It is not recommended to store diluted solutions. Avoid repeated freeze and thaw.

Recommended Usage

Western Blotting (1:1K-5K for neat serum and 1-10 ug/ml for affinity pure using Chemiluminescence technique). An antibody raised to this peptide region has been shown to recognize the 108-110 kDa bands in rat ventral prostrate nuclear extracts and LNCap cells (3).

ELISA (1:10-50K; using 50-100 ng A1R control peptide/well).

Histochemistry & Immunofluorescence: This antibody has been used to localize the rat and human AR (3). We recommend the use of affinity purified antibody at 10-50 mg/ml.

Immuoprecipitation: Not tested. We recommend 5-10 ul neat serum or 1-10 ug affinity pure antibody per 100 ug of protein.

Specificity & Cross-reactivity

The peptide used for making antibodies to rat AR shows 100% homology with human AR. The antibody has been shown to identify the mouse, rat, and human AR. Crossreactivity with other species is not known.

References: 1. Lubahn, D et al (1988) Science 240, 327-330; 2. Chang, C et al (1988) Proc. Natl. Acad. Sci., USA, 85, 7211-7215; 3. Prins, G et al (1991) Endocrinology 129, 3187-3199

"Neat Antisera" are the unpurified antiserum and it is suitable for ELISA and Western.
"Affinity pure" antibodies have been over the antigen-affinity column and recommended for immunohistochemical applications.
"Control peptides" can not be used for Western as they are very short peptides. They are intended for ELISA or antibody competition studies.