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Monitoring genotoxicity
during the photocatalytic
degradation of p-nitrophenol
with VitoTOX on Agilent
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| M. Shani Sekler
1, Y. Levi
1, B. Polyak
1, A. Novoa
1, P. S. M. Dunlop
2, J. A. Byrne
2, R. S. Marks
1 3 * |
1Institute
for Applied Biosciences,
Ben-Gurion University, PO Box
653, Beer-Sheva 84105, Israel
2NIBEC,
University of Ulster at
Jordanstown, Newtownabbey,
Northern Ireland BT37 0QB, UK
3Department
of Biotechnology Engineering,
Ben-Gurion University, PO Box
653, Beer-Sheva 84105, Israel
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| email: R. S. Marks (rsmarks@bgumail.bgu.ac.il) |
*Correspondence
to R. S. Marks, Institute for Applied
Biosciences, Ben-Gurion University, PO
Box 653, Beer-Sheva 84105, Israel.
Funded by:
 EC; Grant
Number: EVKI-CT-2000-00069
| photocatalysis •
genotoxicity • p-nitrophenol
• bioassay |
| p-Nitrophenol is a
common structural unit of many
pesticides and was chosen as a
model compound to monitor
genotoxicity during
photocatalytic degradation. The
genotoxicity of p-nitrophenol
(PNP) and its breakdown products
was measured using a
bioluminescent bacterial
bioassay, VitotoxTM.
The genotoxic potential
decreased with the concomitant
photocatalytic degradation of
the parent PNP concentration.
The rate of genotoxicity
reduction was slower than the
rate of removal of the parent
PNP, due to the formation of
genotoxic by-products. After 6 h
of photocatalytic treatment the
total genotoxicity was removed.
These results indicate that
bioassays can be used as a
simple and highly sensitive
method for monitoring the
general toxicity of chemical
pollutants before, during and
after photocatalytic treatment
or other destructive processes.
Copyright © 2004 John Wiley &
Sons, Ltd. |
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