AFGF 1 H RECOMBINANT (50)
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Product Details: Acidic Fibroblast Growth Factor (FGF 1) human (RECOMBINANT)

Basic Information

 
Product ID: GF-040-4
Product Name: Acidic Fibroblast Growth Factor (FGF 1) human (RECOMBINANT)
Unit: 50µg
Price: 365 Euro
Availability: Available
Category: Growth Factors, Cytokines, Receptors and their Antibodies
Subcategory: Growth Factors/Cytokines/Enzymes

 

Product Details

 
Description: Acidic FGF is a mitogen for cells of mesodermal and neuroectodermal origin, including fibroblasts, endothelial cells, astrocytes, neuroblasts, osteoblasts, and smooth muscle cells. This protein is composed of 140 amino acid residues, and has a 55% homology with basic FGF, including two conserved cysteine residues.

Human Acidic FGF is produced by genetically engineered yeast, and purified by heparin-Sepharose affinity chromatography.

 
Molecular Weight: 15.5 KDa
Biological Activity: Human acidic FGF is mitogenic for a variety of normal cells in culture including fibroblasts, glial cells, vascular endothelial cells.

 
Formulation: 10 mM Tris-HCl, 1.5 M NaCl, pH 7.0 Concentration: 0.50 mg protein/ml.
Purity: Over 95% pure by HPLC analysis and SDS gel electrophoresis.
Storage Conditions: Store at -80°C. It is also possible to keep this protein at low pH (2-5) for a few days.
Stability: Unstable without a carrier protein like gelatin or bovine serum albumin. It is recommended to add some of these proteins, prior to aliquoting, for longer storage.
Temperature: -80°C
References: 1. Gimenez-Gallego et al., Biochem. Biophys. Res. Comm.(1986) 138:611
2. Thornton et. al. (1983), Science 222, 623
3. Gospadorowicz, D. (1987) Methods Enzymol. 147:106