Adenosine deaminase
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Adenosine Receptor Antibodies

The purine nucleoside adenosine modulates diverse physiological functions including induction of sedation, vasodilatation, suppression of cardiac rate and contractility, neurotransmitter release, inhibition of platelet aggregation and lipolysis. Adenosine released from cells interacts with membrane receptors (adenosine receptors, ARs). Based upon, biochemical and pharmacological criteria, ARs have been classified into A1, A2a, A2b, and A3. The high affinity receptor A1 inhibits adenyl cyclase, whereas low affinity receptor A2a stimulates the cyclase via G proteins. A2a receptor is believed to cause vasorelaxation in coronary artery. Various, ARs and their subtypes have recently been cloned from several species. ARs belong to the superfamily of G-protein coupled receptors and predicted to contain 7 transmembrane domains. The N-termini are predicted to be extracellular and the C-termini cytoplasmic. There is an overall 30% homology among the four ARs. ARs are distributed throughout the body. Gene location of various ARs: A1 (Chromosome 1; q.31.3-32.2); A2a (Chromosome 22); A2b (Chromosome 17; p.11.2-12); A3 (Chromos.1).

Adenosine deaminase (ADA; EC 3.54.4) catalyzes deamination of adenosine to inosine. ADA is found in primarily cytosolic. It has also been located at the cell membrane and implicated in the control of the extracellular concentration of adenosine.

ADI has prepared antibodies to adenosine receptors using subtype specific peptide sequences. Because of sequence homology of a given sequence, many antibodies show interspecies crossreactivity for each receptor subtype. However, there is no substantial sequence homology of the selected antigenic peptides between different adenosine receptor subtypes. Antibody crossreactivity is based upon either known reactivity of antisera with other species receptor subtypes or based upon high degree of sequence homology. Antibodies to ADA are also available to study the role of ADA in ARs structure and function.

 

 Items  Antigen peptide location   Antibody Host   Ab Crossreactivity  Neat Antisera
Cat #
(100 ul)		 Aff. Pure Ab
Cat #
(100 ug)		  * Control Peptide Cat# (100 ug)   **WB +ve Control Cat #
 
A1 R, 14 aa, EC3 Rb R, H, Rb, C, B  A1R11-S  A1R11-A A1R11-P  
A2a C, 30 aa, IC4 Rb R, H, B, C A2aR21-S A2aR21-A A2aR21-P A2aR21-C
 hA2b H, 16 aa, EC2 Rb H, M, R A2bR23-S A2bR23-A A2bR23-P  
rA3 R, 15 aa, EC3 Rb R A3R31-S A3R31-A A3R31-P  
A3 (Ab#1)  H, 15 aa, EC3 Rb H  A3R32-S  A3R32-A A3R32-P  
 A3 (Ab #2) H, 15 aa, EC3 Ch H A3R33-S A3R33-A A3R33-P  
ADA  bADA Rb  M, R, H, B  ADA11-S      
 Control Rabbit IgG  For use in ELISA, Western, Immunohisto. 20009-1 (1 mg) 
 Control Chicken IgG  For use in ELISA, Western, Immunohisto.  20010-1 (1 mg)



M= Mouse; R=Rat; H=Human; Ha=Hamster; Rb=Rabbit; B=Bovine; C=Chicken; CT= near C-terminus; NT=near N-terminus; Internal=Middle of protein. EC=Extracellular domain; IC=Intracellular domain;

"Neat Antisera" are the unpurified antiserum and it is suitable for ELISA and Western.
"Affinity pure" antibodies have been over the antigen-affinity column and recommended for immunohistochemical applications.
"Control peptides" can not be used for Western as they are very short peptides. They are intended for ELISA or antibody competition studies.