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Fatty Acid Binding Proteins
(FABPs) Antibodies
Fatty acids are important for
general cellular metabolism. A number of proteins have been implicated in the
transport and storage of fatty acid. FABPs (fatty acid binding proteins) are a
group of cytoplasmic, small mol wt (14-15 kDa) and proteins that has widespread
tissue distribution. FABPs are quite abundant (3-5% of total cellular protein).
At least 7 FABPs, FABP1-7, have been cloned and characterized from various
tissues. FABPs can bind long-chain fatty acid, fatty-acid acyl-CoA and
acyl-L-carnitine. Several different isoform of FABP have been identified and
generally referred to by tissue type (liver, heart, intestine, adipocyte,
kidney, brain etc; protein designated as H-FABP indicates that it is heart
type). However, expression of these isoform is not exclusive and more than one
isoform can be found in a given cell or tissue. Three main types of FABPs that
were initially discovered in the heart (FABP-H), liver (FABP-L), and intestine
(FABP-I) are not exclusive these tissues and show considerable differences at
the amino acid level (~30% identity). Other FABPs recently detected in
adipocyte, kidney, and brain show a high degree of sequence homology among each
other and with other FABPs. FABPs are also known as mammary derived growth
inhibitor (MDGI), adipocyte lipid binding protein (ALBP), Myelin protein P2
homolog, P2 adipocyte protein, 422 protein (P15).
Human FABP-H or adipocyte is
132-aa protein (chromosome 2p11) Rat and mouse adipocyte-FABPs are 133 aa single
polypeptide chains.
| Items |
Antigen
peptide location |
Antibody
Host |
*Expected
Ab Crossreactivity |
Antiserum
Cat #
(100 ul) |
Aff.
Pure IgG
Cat #
(100 ug) |
* Control Peptide
Cat#
(100 ug) |
FABP-H/
A-FABP |
h, 12 aa ~CT |
Rb |
h, r, m, b |
FABP11-S |
. |
FABP11-P |
|
FABP-H/
|
Purified human
FABP-heart protein for WB +ve control (in SDS-PAGE sample buffer)
Cat # FABP11-C (100 ul)
|
FABP-H/
A-FABP |
r, 20 aa ~CT |
Rb |
m, r, h
|
FABP12-S |
. |
. |
FABP-H/
A-FABP |
h, FABP-H |
m,
mono |
h (m, r?) |
. |
FABP13-M |
. |
|
Control Rabbit IgG |
For use in ELISA, Western, Immunohisto,
Cat # 20009-1 (1 mg)
|
|
Control Mouse IgG
|
For use in ELISA, Western, Immunohisto,
Cat # 20008-1 (1 mg)
|
m=mouse; r=rat; h=human; b=bovine; d=dog; ~CT or ~NT=near
C or N-terminus. EC=Extracellular;
CP=Cytoplasmic domain;
* Expected antibody crossreactivity information is
mostly based upon high (>70%) sequence conservation of antigenic/control
peptides in various species. When antibody crossreactivity has actually been
experimentally confirmed in various species, it will be mentioned in the
appropriate data sheets.
"Neat Antisera or antisera" are the unpurified
antiserum and it is suitable for ELISA and Western.
"Affinity pure" IgG may be more suitable for
immunohistochemical (IHC) applications and to reduce background in most
immunological applications including ELISA and Western.
"Control peptides" can not be used for Western as
they are very short peptides. They are intended for ELISA or antibody blocking
studies to establish antibody specificity.
Western blot +ve protein controls, where available,
are semi-pure, pure or recombinant proteins that are formulated in SDS-PAGE
sample buffer (reduced). They are recommended to be used for Western (load 10
ul/lane) for visulization with antibodies
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