DESCRIPTION
The study of cell proliferation and cell viability requires the accurate
quantification of the number of viable cells in a cell culture. Therefore,
assays for calculating cell viability are necessary for optimizing cell
culture conditions, evaluating cell growth factors and nutrients,
discovering novel antibiotics and anti-cancer drugs, evaluating toxic
effects of environmental pollutants and cell mediated toxicity and studying
programmed cell death (apoptosis).
The CellQuanti-MTT kit provides a convenient, sensitive, quantitative and
reliable assay for determining the number of viable cells in a given
culture. This homogeneous colorimetric assay is based on the conversion of a
tetrazolium salt MTT, a pale yellow substrate, to formazan, a purple dye.
This cellular reduction reaction involves the pyridine nucleotide cofactors
NADH and NADPH and is only catalyzed by living cells. The formazan product
has a low aqueous solubility and is present as purple crystals. Dissolving
the resulting formazan with a solubilization buffer permits the convenient
quantification of product formation, which is directly proportional to the
number of living cells, by measuring absorbance in the range of 550 - 620 nm
with a plate reader.
Reagents in the kit have been carefully formulated and optimized for
sensitivity, assay robustness and automation.
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