11-Dehydrothromboxane B2 RIA test*
Home Up Calcitonin IRMA test Anti-hTG (human thyroglobulin) RIA test TG (thyroglobulin) IRMA test Anti-TPO (thyroid peroxidase) RIA test Turbo tsh 1 Turbo tsh 2 Turbo tsh3 Turbo tsh4 Turbo tsh5 TSH ELISA test Free T4 (thyroxine) RIA test Free T4 Blue T4 (thyroxine) RIA test T4 (thyroxine) RIA test 1 T4 (thyroxine) RIA test 2 T4 (thyroxine) RIA test 3 Free T3 (triiodothyronine) RIA test T3 (triiodothyronine) RIA test T3 (triiodothyronine) RIA test 1 T3 (triiodothyronine) RIA test 2 T3 (triiodothyronine) RIA test 3 Progesterone RIA test Testosterone RIA test Cortisol RIA test DHEA-SO4 (dehydroepiandrosterone sulphate) RIA test hGH (human growth hormone) IRMA test   a hGH (human growth hormone) IRMA test 1  b hLH (luteinizing hormone) IRMA test hLH (luteinizing hormone) IRMA test 1 h FSH (follicle stimulating hormone) IRMA test h FSH (follicle stimulating hormone) IRMA test 1 h Prolactin IRMA test h Prolactin IRMA test 1 Insulin RIA test C-peptide IRMA test SHBG (sex hormone binding globulin) IRMA test AFP (alpha fetoprotein) IRMA test AFP (alpha fetoprotein) IRMA test 1 AFP (alpha fetoprotein) ELISA test 2 ß hCG (beta human chorionic gonadotropin) IRMA test ß hCG (beta human chorionic gonadotropin) IRMA test 1 ß hCG (beta human chorionic gonadotropin) ELISA test 2 hCG (human chorionic gonadotropin) free ß-subunit test 3 h CEA (carcinoembryonic antigen) IRMA test PSA (prostate specific antigen) IRMA test PSA (prostate specific antigen) IRMA test 1 Free PSA (prostate specific antigen) IRMA test PSA (prostate specific antigen) ELISA test Prostaglandin D2 RIA test 8-iso-Prostaglandin F2a RIA test Bicyclo Prostaglandin E2 RIA test 11-Dehydrothromboxane B2 RIA test* 13,14-Dihydro-15-keto-PGF2a RIA test* 6-keto-PGF1a /2,3-dinor-6-keto-PGF1a RIA test Prostaglandin E2 RIA test Prostaglandin F2a RIA test* Thromboxane B2/2,3-dinor-TxB2 RIA test

 

 

11-dehydro-Thromboxane B2 radioimmunoassay kit (RK-67M)

Intended use:

Thromboxane B2, the stable hydrolysis product of thromboxane A2, is further metabolised to produce 11-dehydro-Thromboxane B2 (11-dehydro-TXB2). In plasma and urine the concentration of 11-dehydro-TXB2 rather than that of TXB2, is accepted to be the relevant parameter of systemic thromboxane production. Extrarenal thromboxane biosynthesis can be most reliably monitored by measuring urinary 11-dehydro-TXB2 concentration. This is clearly assisted by setting up the present RIA system, which combines a high-specific-activity radioiodinated tracer with a sensitive polyclonal rabbit antiplasma. The assay has been thoroughly optimised for determination of urinary 11-dehydro-TXB2 - this is permitted by the elaboration of such a unique selective solid-phase extraction procedure that abolish the requirement of chromatographic purification step.

Assay characteristics:

Assay type:   RIA
Isotope:   125I
Separation:   Magnetic solid phase
Sample type:   Human urine
Sample preparation:   Solid-phase extraction without chromatography
Sample volume:   1 ml
Sample capacity:   40 (duplicate) or 24 (triplicate) unknowns
Time:   One working day
Hands-on time:   3 hours

Special advantages:

- Good sensitivity fitting to urinary concentrations
- Robust and precise assay protocol
- Reasonably short hands-on time
- Reconstituted reagents can be stored for re-use
- Detailed up-to-date theoretical background of thromboxane metabolism
- Homogeneous immunoreactivity obtained with unique solid-phase extraction procedure

Availability: On request

Pack contents:

- 125I-methyltyrosyl-11-dehydro-thromboxane B2 Tracer, in organic solution
- 11-dehydro-thromboxane B2 Standard, 10 ng/ml, lyophilised
- 11-dehydro-thromboxane B2 rabbit Antiserum, lyophilised
- Assay Buffer concentrate
- Magnetizable latex, ready to use
- Full instructions booklet

Assay parameters:

Measuring range:   1.23 - 300 pg/tube  
Typical IC50-value: ~ 25 pg/tube at 50% B / B0  
Sensitivity: ~ 2 pg/tube  
Specificity: See cross reactions below:  
  Thromboxane B2 0.006
  6-keto-PGF1a < 0.010
  Prostaglandin E2 < 0.001
  Prostaglandin F2a < 0.001
  Prostaglandin D2 0.500
  Prostaglandin A2 < 0.001
  Prostaglandin B2 < 0.001
  Prostaglandin F1a < 0.001
  13,14-dihydro-15-keto-PGE2 < 0.001
  13,14-dihydro-15-keto-PGF2a 0.001
  13,14-dihydro-15-keto-PGA2 < 0.010
  13,14-dihydro-6,15-diketo-PGF1a < 0.001
  6-keto-PGE1 < 0.001
  11-dehydro-TXB2 100.00
  2,3-dinor-TXB2 0.040
  2,3-dinor-6-keto-PGF1a < 0.010
  o-hydroxy-hippuric acid < 0.001

11-Dehydrothromboxane B2 RIA test*