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| |
11-dehydro-Thromboxane B2 radioimmunoassay kit (RK-67M)
|
Intended use:
Thromboxane B2, the stable hydrolysis product of thromboxane
A2, is further metabolised to produce 11-dehydro-Thromboxane
B2 (11-dehydro-TXB2). In plasma and urine the
concentration of 11-dehydro-TXB2 rather than that of TXB2,
is accepted to be the relevant parameter of systemic thromboxane
production. Extrarenal thromboxane biosynthesis can be most reliably
monitored by measuring urinary 11-dehydro-TXB2 concentration.
This is clearly assisted by setting up the present RIA system, which
combines a high-specific-activity radioiodinated tracer with a sensitive
polyclonal rabbit antiplasma. The assay has been thoroughly optimised
for determination of urinary 11-dehydro-TXB2 - this is
permitted by the elaboration of such a unique selective solid-phase
extraction procedure that abolish the requirement of chromatographic
purification step.
Assay characteristics:
|
Assay
type: |
|
RIA |
|
Isotope: |
|
125I |
|
Separation: |
|
Magnetic solid phase |
|
Sample
type: |
|
Human
urine |
|
Sample
preparation: |
|
Solid-phase extraction without chromatography |
|
Sample
volume: |
|
1 ml |
|
Sample
capacity: |
|
40
(duplicate) or 24 (triplicate) unknowns |
|
Time: |
|
One
working day |
|
Hands-on time: |
|
3
hours |
Special advantages:
|
- |
Good sensitivity fitting to urinary concentrations |
|
- |
Robust and precise assay protocol |
|
- |
Reasonably short hands-on time |
|
- |
Reconstituted reagents can be stored for re-use |
|
- |
Detailed up-to-date theoretical background of
thromboxane metabolism |
|
- |
Homogeneous immunoreactivity obtained
with unique solid-phase extraction procedure |
Availability:
On request
Pack
contents:
|
- |
125I-methyltyrosyl-11-dehydro-thromboxane B2
Tracer, in organic solution |
|
- |
11-dehydro-thromboxane B2 Standard, 10 ng/ml,
lyophilised |
|
- |
11-dehydro-thromboxane B2 rabbit Antiserum,
lyophilised |
|
- |
Assay Buffer concentrate |
|
- |
Magnetizable latex, ready to use |
|
- |
Full instructions booklet |
Assay parameters:
|
Measuring range: |
|
1.23 - 300 pg/tube |
|
|
Typical IC50-value: |
~ 25 pg/tube at 50% B / B0 |
|
|
Sensitivity: |
~ 2 pg/tube |
|
|
Specificity: |
See cross reactions below: |
|
| |
Thromboxane B2 |
0.006 |
| |
6-keto-PGF1a |
< 0.010 |
| |
Prostaglandin E2 |
< 0.001 |
| |
Prostaglandin F2a |
< 0.001 |
| |
Prostaglandin D2 |
0.500 |
| |
Prostaglandin A2 |
< 0.001 |
| |
Prostaglandin B2 |
< 0.001 |
| |
Prostaglandin F1a |
< 0.001 |
| |
13,14-dihydro-15-keto-PGE2 |
< 0.001 |
| |
13,14-dihydro-15-keto-PGF2a |
0.001 |
| |
13,14-dihydro-15-keto-PGA2 |
< 0.010 |
| |
13,14-dihydro-6,15-diketo-PGF1a |
< 0.001 |
| |
6-keto-PGE1 |
< 0.001 |
| |
11-dehydro-TXB2 |
100.00 |
| |
2,3-dinor-TXB2 |
0.040 |
| |
2,3-dinor-6-keto-PGF1a |
< 0.010 |
| |
o-hydroxy-hippuric acid |
< 0.001 |
|
11-Dehydrothromboxane B2 RIA
test*
|
